The National Programme on Conservation and Utilization of Microbial Genetic Resources and Invertebrates of Agricultural Importance

Collection of Agriculturally Important Fungi
• History
The collection was found in the 90-thies of the twenty century. Since this time Dr. Jiřina Krátká, Dr. Eliška Sychrová and Dr. Michaela Zemánková participated on the direction and focusing of it. Dr. David Novotný is the current head and curator of the collection.

• Characterization of collection
phytopathogenic and potentially phytopathogenic, mycotoxinogenic and potentially mycotoxinogenic fungi (except biotrophic species), edible and medicinal mushroom grown by Czech farmers, about 300 strains, especially from groups Deuteromycota, Ascomycota a Basidiomycota, antibodies against some phytopathogenic fungi – about 15 items

• Activities, services
collect, maintain and preserve cultures
supply cultures
provide information on strains in the collection
conservation fungi genetic resources
consultation services on cultivation, isolation, identification and preservation of filamentous fungi
diagnostic, microbiological, morphological physiological and biochemical characterization of phytopathogenic, entomopathogenic, nematophagous, mycotoxinogenic and endophytic fungi important for agriculture and safety agriculture commodities and foodstuff (especially fungi of fruit and forest trees, energetic crops, cereals, edible and medicinal mushrooms)

• Methods of culture maintenance
storage under mineral oil on an agar slant; in low temperature

Supply of cultures of filamentous fungi
Identification of filamentous fungi, including isolation and purification of cultures
Expert opinion activities in ecology of microscopic fungi
Deposit of strains of filamentous fungi subject to patent application according to the national rules
Safe deposit of customers´ cultures

Crop Research Institute Prague
Drnovská 507
161 06 Praha 6 – Ruzyně


RNDr. David Novotný PhD. (curator), 233022373, 233022358
Jitka Dunaiová

Collection of Biotrophic Fungi
• History
Determination of physiologic races of wheat rusts started in the sixties of the last century. Results were periodically published in scientific journals (Genetika a šlechtění, at present Czech Journal of Genetics and Plant Breeding or in Ochrana rostlin, at present Plant Protection Science). Results of race surveys were discussed with wheat breeders and applied in the wheat breeding for rust resistance. Since that time determination of rust races (pathotypes) is being carried out till now and isolates are stored.

• Characterization of collection
The collection comprises 826 leaf rust isolates, 29 stem rust isolates, 2 proveniences of yellow rust and 15 isolates of powdery mildew.

• Activities, services
The rust isolates are used in the tests for resistance in the field as well as in the greenhouse. Rust inoculum is used by domestic and also foreign breeders and institutions.

• Methods of culture maintenance
Determined rust isolates are kept in collections as urediospores. Powdery mildew is kept on leaf segments. Isolates most frequently applied are kept in a refrigerator at 5°C, the core collection is kept at ultra low temperature at -85°C.

• Systems of quality
Determination of rust isolates is carried our on international sets of differentials. It is based on determination of single pustule isolates. Approved methods are used for storing the rust and powdery mildew isolates.

• Involvement to national and international organizations/platforms
Rust isolates have been used in international scientific collaboration (Australia, U.K., France, Hungary, and Slovakia). At the domestic level accessions of the collection are used by plant breeders and by the Central Institute for Supervising and Testing in Agriculture and a similar institution in Slovakia.

• utilization of strains
Isolates are used in the wheat breeding as well as resistance testing both in the Czech and Slovak Republics. They are used by the Central Institute for Supervising and Testing in Agriculture and the. They are also used in the research work on national and international level.

• users, clients
Plant breeding companies
Central Institute for Supervising and Testing in Agriculture
Central Controlling and Testing Institute in Agriculture in Slovakia
Foreign scientific institutes

Crop Research Institute
Division of Plant Genetics, Breeding and Product Quality
Drnovská 507
161 06 Praha 6 – Ruzyně
Czech Republic

Mgr. Alena Hanzalová, Ph.D. (curator)
e-mail:, tel: 233022243
Ing. P. Bartoš, DrSc

Collection of Edible and Medicinal Macromycetes
• History
Collection was established in 2006 as a collection of diaspores – spores, sclerotia, etc., but mostly sporeprints, with the goal to create the original set of accessions of edible and/or medicinal species of fungi autochthonous for the Central Europe. Since 2008, the collection has been a part of the National Programme on Protection of Genetic Resources of Economically Significant Microorganisms and Tiny Animals.

• Characterization of collection
Our goal is to create a specialised mycological collection focused on acquisition and conservation of genetically homogenous cultures of autochthonous species of macromycetes potentially important for both private and commercial cultivations, and on support of newly developing horticultural mycotechnologies. Recently, totally 130 strains are included in the collection. The species of family Morchellaceae are the most numerous, the other strains belong to edible and/or medicinal Basidiomycetes.

• Methods of culture maintenance
Isolates are maintained as mycelial cultures on rye grains or malt extract agar with glucose in refrigerator, and under cryopreservation in the Central Laboratory of the National Programme in Praha-Ruzyně.


Centre of the Region Haná for Biotechnological and Agricultural Research
Department of Genetic Resources for Vegetables, Medicinal and Special Plants
Crop Research Institute
Šlechtitelů 29, 783 71 Olomouc

Contact persons:
Curator: Dr. Irena Petrželová, Ph.D.
CRH for Biotechnological and Agricultural Research
Dept. of Genetic Resources for Vegetables, Medicinal and Special Plants
Crop Research Institute
Šlechtitelů 29, 783 71 Olomouc
Phone: 585208966
Mob.: 702087798
Culture Collection of Dairy Microorganisms Laktoflora®
• History
The Culture Collection of Diary Microorganisms (CCDM) Laktoflora® was established in 1965 as a gen-bank of bacteria, yeast and fungi for dairy research, agriculture, medicine and food processing.

• Characterization of collection
Laktoflora® collection now maintains about 550 strains and 700 isolates of bacteria, yeast and fungi. The bacteria are represent by 540 isolates belong to 150 bacterial species. Multispecies isolates for dairy farming and food processing includes 172 cultures and represents special and unique part of collection. The collection preserves 60 isolates of 20 species belong to ascomycetous yeast mainly. The fungal organisms are represented by 95 isolates of Penicillium camemberti, P. nalgiovense and P. roqueforti primarily.

• Methods of culture maintenance
The isolates are deposited in lyophilized form, deep-frozen and/or cultured on media. The collection is registered in WFCC No 878 belong to acronym CCDM. The operation and maintenance of the collection is funded by The Czech National Programme on Conservation and Utilisation of Plant genetic resources and Agrobiodiversity. The collection co-operates closely with Dairy Research Institute Ltd. on various research projects funded especially by The Ministry of Agriculture of the Czech Republic and The Technological Agency of the Czech Republic. The important participants of project are other RTDs and SMEs as well.
The Laktoflora’s research is focused on the isolation of new strains, genetic and phenotypic identification, re-identification and characterization of bacterial, yeast and fungal strains. Prebiotic and probiotic effect of bacterial and fungal strains on human health is tested under laboratory conditions (for example production of biocines, assimilation of cholesterol, production of exopolysacharides, resistance to antibiotics) as well as in pilot operations (sourdough, milk products etc). The fundamentals of our collection are the inquiry of microorganisms and fungi, their maintenance, recovery as well as identification and re-identification based on biochemical, morphological and molecular/genetic methods. The service provided by the collection is in accord to the activity and equipment of the collection
Ing. Petr Roubal, CSc.
Miloslava Kavková, Ph.D. (curator)
Culture Collection of Dairy Microorganisms
Dairy Research Institute
Soběslavská 841
390 02 Tábor
Tel./Fax: 381 252 980
Mobil: 723679012
Collection of Brewery Microorganisms
• History
The collection of brewer’s yeast was established in 1946 as part of a collection of yeasts and yeast-like microorganisms under the leadership of Dr. Kocková-Kratochvílová, and has operated independently since 1953. Since 1964 the collection has been a member of the Federation of Czech and Slovak Collections of Microorganisms and is internationally registered as RIBM under the number 655. By focusing on the production strains of brewing yeast it is unique in Central and Eastern Europe. In 1996 the brewer’s yeast collection became (as one of very specifically targeted and industrially applicable collections) part of the „National Program of the Gene Pool of Microorganisms and Small Animals of Economic Importance and their Use in Reference Diagnostics.“ This project is also supported by the Czech government in the form of a grant by the Ministry of Agriculture. The program is led by the Council of Microbial Genetic Resources, which monitors the mandatory retention of a functional existence of collections of microorganisms of a corresponding character.

• Characterization of collection
RIBM collection includes two separate collections, collections of brewer’s yeast and collection of bacteria and wild and wine yeast. The collection currently includes a total of 308 strains of yeasts and bacteria. The most important part of the collection consists of a collection of 118 strains of brewer´s yeast Saccharomyces cerevisiae and S. pastorianus, collected continuously since 1953 from Czech and foreign breweries. A collection of wild and wine yeasts contains 79 strains classified into genera Saccharomyces, Torulaspora, Zygosaccharomyces, Dekkera, Williopsis, Pichia, Schizosaccharomyces, Saccharomycodes, Candida, Kloeckera and Rhodotorula. Collection of bacteria houses 108 strains of Lactobacillus, Leuconostoc, Pediococcus, Tetragenococcus, Lactococcus and three strains of genus Pectinatus.

• Methods of culture maintenance
Collections of brewing and wild yeasts are kept on agar slants under paraffin-covered cotton wool plugs and simultaneously on wort agars overlayered with sterile paraffin oil separately in a refrigerator. These best ways of keeping the cultures allow the rapid delivery to a customer of a strain in an active state on an agar slant or fermenting in 1.5 liters of wort, which facilitates the transfer of the production strain into production. Since 2006 strains of brewer’s yeast are stored in cryo-tubes with protective medium in liquid nitrogen at -196 ° C. Storing in liquid nitrogen (cryopreservation) is considered optimal for the long term preservation of yeast in a viable state. Lactic acid bacteria are stored in parallel freeze-dried in skimmed milk in liquid nitrogen.
Use of the collection:
Collection strains are primarily used for research projects of RIBM and other research organizations (MSM6019369701, 1M0570, 2B08022 2A-2TP1 / 031, FI-IM5 / 067, QF3299, QI91B226 etc.). The strains are also used for teaching and diploma and doctoral works at universities.

Research Institute of Brewing and Malting
Lípová 15
120 44 Prague 2

• curator
RNDr. Dagmar Matoulková, Ph.D.
phone.: 224 900 132
• others
Ing. Petra Kubizniaková
phone.: 224 900 152


Collection of Industrially Utilizable Microorganisms

• Characterization of collection (strains), the number of cultures
The collection contains bacteria, yeasts and fungi of food industry and agriculture importance (total of 150 strains – 125 strains of yeasts, 17 strains of bacteria and 8 strains of fungi).

• Methods of culture maintenance
The strains were kept on agar slants in an active state.

• Activities, services
The collection covers the needs in the field of food and agriculture. Strains are used for processing food and agricultural commodities, for the purposes of controlling analytical work and research projects solved in FRIP and elsewhere.

• Application of strains
The largest group of microorganisms in the collection are yeast – 125 strains. These are the strains of the alcoholic fermentation and strains for production of baker´s and feed yeasts used in the relevant fields, particularly in research. Furthermore are in the collection strains which are used for special production, e.g. strains able to dispose of petroleum materials and strains capable of producing a nutritionally useful chelates of essential trace elements and strains which are used for special dietetics.

The second group of microorganisms in the collection are bacteria – 17 strains. Some of them are used for microbiological methods of analyses, others are used for testing innovative food antibacterially active.

The third group of mircroorganisms in the collection are fungi – 8 strains. Most of these strains are producers of enzymes that are used in the food industry and agriculture. They are amylases, glucose oxidase, cellulases and amyloglucosidase.

• Customers
Users of collection are mainly research institutes working in the field of microbiology, food production and agriculture. An example of such a client are Laboratory of enzyme technology MBÚ Czech Republic, Prague, Laboratory of Biology and Microbiology – Institute of Landscape Ecology of the ASCR, Czech Budejovice, Research Institute of Brewing and Malting, Research Institute antibiotics and biotransformation Prague and others.

Food Research Institute Prague
Ing. Zdeněk Moučka (curator)
tel.: 737 287 009
Ing. Marian Urban, Ph.D.
tel.: 737 287 003

Collection of Phytopathogenic Microorganisms PUOC
Information on collection:
• History
The collection of fungal microorganisms pathogenic to plants has been formed by Prof. Lebeda from 1977, during his work in Plant Breeding Station of Sempra company in Smržice. Following his employment (from 1994) at the Dept. Botany, Faculty of Science, Palacký University in Olomouc the collection was enlarged by isolates of cyanobacteria and algae (administered by Dr. Hašler and managed by prof. Poulíčková) and collection of viruses and phytoplasmas (managed by Prof. Navrátil at Department of Cell Biology and Genetics). The UPOC collection has expanded by expeditions organized yearly to collect new samples.

• Characteristic of collection (strains), number of cultures
The national database include
177 isolates of 15 species of phytopathogenic fungi and fungi-like organisms
31 strains of 31 species of cyanobacteria (12) and algae (7)
5 isolates of 4 species of phytoplasmas and 28 isolates of 7 virus species
others are part of working collection.

• Activities/research activities of Department of Botany
Genetic variability of plant pathogenic fungi;
Biodiversity and genetic variability of host-pathogen interactions in crop and wild plant pathosystems;
Mechanisms of resistance in host-pathogen interactions;
Plant breeding (classical and biotechnological approaches) in disease resistance;
Tests of plant pathogen for fungicide resistance;
Biology, ecology and genetics of cyanobacteria and algae
Epidemiology, biology and genetics of phytoplasmas and viruses

• Services
providing samples for non-commercial purposes
consultancy and determination
methods of culture maintenance see methodology (in Czech language)
quality systems, internal norms, correct laboratory practice
according to requirements of the National programme

• Involvement to national and international organizations/platforms
IBEB (International Bremia Evaluation Board)
• international cooperation
interchange of isolates, developing methods for storage, cultivation and testing of host plants resistance
• catalogue – on www VURV Praha

Utilization of collection:
• Utilization of strains
teaching, research, screening of plant resistance, reference strains for phytosanitary diagnostics, State phytosanitary administration and Central Checking and Testing Agricultural Institute, consultancy activities and postgraduate teaching of professional staff of water management institutions/authorities

• Users, clients
students, teachers, scientists, specialists of State Phytosanitary Administration, breeding companies and water authorities, …

Microorganisms in UPOC collection

Palacký University in Olomouc
Faculty of Science
Department of Botany
Šlechtitelů 11
783 71 Olomouc-Holice

Staff, contacts:
• head/responsible person
Prof. Ing. Aleš Lebeda, DrSc. (curator)
• others
Doc. RNDr. Michaela Sedlářová, Ph.D.
Mgr. Pavla Šiková
RNDr. Barbora Mieslerová, Ph.D.
RNDr. Božena Sedláková, Ph.D.
RNDr. Petr Hašler, Ph.D.
Prof. RNDr. Milan Navrátil, CSc.
• contact: e-mail, telephon, fax: 585634824, www 585634800 585634809
fax 585634824

Culture Collection of Basidiomycetes
• History
Culture Collection of Basidiomycetes Important for Agriculture (CCBAS-A) is an integral part of the mother Culture Collection of Basidiomycetes (CCBAS), which was established in 1959 at the Institute of Microbiology AS CR in Prague. Since 2003 the CCBAS-A is incorporated in the National Programme of Protection and Utilization of Genetic Resources of Microorganisms and Tiny Organisms Economically Significant for Agriculture. At present it includes 346 strains of basidiomycetes belonging to 168 species, mostly wood-rotting, and is continually extended by adding new verified strains.

• Characterization of Collection
potential to decompose polutants (organic compound) with their enzymes
metabolize pesticides thanks to extracellular enzyme lacasa
thus help with bioremediation of contaminated soil
332 isolates

• Activities/research activities
The main goal of the work of the collection staff is to develop alternative and to optimize current procedures of cryopreservation and other methods of maintenance of fungal cultures. These methods are then verified and introduced into practice. A new original method of cryopreservation of fungal cultures on perlite was developed and verified in our laboratory. This method is now used for the preservation of fungi not only in our, but also in many other collections in the world. Our cultures can serve as a reference material for further taxonomic, phylogenetic and other studies including studies of their biotechnologically exploitable properties. They are plentifully utilized by home and foreign research scientists. Another activities lie in consultations regarding cultivation, physiology and genetics of basidiomycetes and publishing of the achieved results in impacted international journals. Dr. Homolka takes part in home and international conferences and congresses and gives lectures at the Faculty of natural sciences (Essentials of physiology and genetics of filamentous fungi). He is also engaged in the training the staff of the collections in database operation.

• Methods of culture maintenance
The cultures are maintained by two independent principal methods (subculturing on solid agar media and cryopreservation on perlite) to preserve their quality and quantity. The crucial characteristics of the cultures are recorded in the database of the National Programme. The cultures on perlite can be distributed directly without reinoculation and reused for successive inoculations. They also turned out to be a good substitute for agar cultures in long-term maintenance of fungi.
The viability of the cultures and their crucial characteristics (macro- and micromorphology, growth rate, purity) is tested every year in case of agar cultures and every second to fifth year in case of perlite cultures. If needed, the specific characteristics are tested, e.g. enzymatic or genetic ones.
The data on cultures and collection operation are stored in a local operation database (developed especially for this purpose) and also in a central database of the National Programme.

• Database
involvement to national and international organizations/platforms
CCBAS is a member of Federation of Czechoslovak Collections of Microorganisms (FCCM) and World Federation of Culture Collections (WFCC), where it is listed under the number 558. In the frame of the international cooperation and information exchange, our collection provides the interested persons with the data on the maintained basidiomycete cultures (see the databases of NP, FCCM or WFCC) and on the basis of order also cultures to abroad. CCBAS cooperates with home and renowned foreign culture collections (e.g. CBS at Utrecht or MUCL at Louvain-la-Neuve) and universities (e.g. at Wageningen or at Oslo).
As mentioned above, the strains are used predominantly for noncommercial research and teaching purposes.

Institute of Microbiology of the Academy of Sciences of the Czech Republic
Laboratory of Environmental of Microbiology
Vídeňská 1083
142 20 Prague 4
Czech Republic

• head and curator
RNDr. Ivana Eichlerová, PhD,
• others
Ing. Petra Dobiášová

tel.: 241 062 397
fax: 241 062 384

web CCBAS:
Collection of Hop Pathogens
• History
The collection was established in 2003. The main attention is paid to hop pathogens – viruses and viroids.

• Characterization of collection
8 virus isolates
1 fungus isolate

• Methods of culture maintenance
infected hop plant, CaCl2, lyophilization

• Control
Each year monitoring is performed pathogens control by ELISA and RT-PCR, the same time there is a continuous obtaining of new isolates
Isolates: ApMV, ArMV, HLV, HMV, HLVd
Diagnosis of pathogens – ELISA, PCR, phytopathological – virology

Utilization of strains
positive controls for testing of nursery material
providing of strains for research

The Central Institute for Supervising and Testing in Agriculture (CISTA)
The Czech University of Life Sciences Prague (CULS), Institute of Plant Molecular Biology – The Biology Centre of the Czech Academy of Sciences, České Budějovice

Hop Research Institute Zatec
Ing. Petr Svoboda, CSc., curator
+420 415 732 121
fax +420 415 732 150
Culture Collection of Fungi
• History
Established in 1964-65 under the leadership of Dr. Olga Fassatiová.
In 2006, part of Culture Collection of Fungi was incorporated into the Czech National Programme of Protection of Genetic Resources of Economically Significant Microorganisms and Tiny Animals (NPPGR).

• Characteristic of collection (strains), number of cultures
Culture collection maintains saprotrophic filamentous microfungi, predominantly Ascomycota a Zygomycota.
Under the Czech National Programme, 322 fungal strains are maintained (of total 4000 strains).
In database of the Czech National Programme the following fungi are incorporated: food contaminants, toxigenic fungi, plant pathogenic fungi, entomopathogens and fungi of biotechnological potential.

• Research activities
Taxonomy of ascomycetes
Biodiversity and ecology of soil and leaf litter microfungi
Association of fungi, bark beetles and trees
Clinically important fungi
Fungi in our environment (e.g. air, food, flats)

• Services
Supplying of fungal strains under the Czech National Programme free of charge for research and teaching purposes.

• Charged services
Supplying of strains for both inland and foreign researchers and institutions for teaching and research purposes. The cultures are distributed on agar slants or in lyophilized form.
Safe deposition of fungal strains.
Freeze-drying of fungal strains.
Identification of pure and mixed fungal cultures.
Molecular analyses of fungi.

• Methods of culture maintenance
In tubes on slope agar media in a refrigerator at approx. 5 oC
Freeze-drying (skim milk as a protective medium)
Alginate pellets

• Involvement to national and international organizations/platforms
World Federation for Culture Collections (WFFC) – since 1972 under No. 182
European Culture Collections Organization (ECCO) – since 1985
Federation of Czechoslovak Collections of Microorganisms) (FCCM)

Utilization of collection:
Maintenance of fungi for teaching and research purposes
Supplying of strains for non-profit institutions and industrial laboratories
Long term maintenance of microfungi important for science.
Atlas of zygomycetes(2009) (in Czech)
Collection of Food Relevant Microscopic Fungi under the Czech National Programme of Protection of Genetic Resources of Economically Significant Microorganisms – A Short Report

Charles University in Prague
Faculty of Science
Department of Botany
Culture Collection of Fungi (CCF)
Benátská 2
CZ – 128 01 Prague 2
Czech Republic

RNDr. Alena Kubátová, CSc. (curator)
Mgr. Vít Hubka, Ph.D.
Mgr. Miroslav Kolařík, Ph.D.
Mgr. Ondřej Koukol, Ph.D.
Adéla Kovaříčková
Petra Seifertová

Phone: +420 221951656 (Dr. A. Kubátová)
Fax: +420 221951645
Web page:

Czech Collection of Phytopathogenic Oomycetes
The collection was established in 2005 under the leadership of Karel Cerny and was admitted in 2012 as a member of The National Programme on Conservation and Utilization of Genetic Resources of Microorganisms.

• Characterization of collection
There are approx. 312 strains of approx. 29 oomycetous taxa preserved which are belonging to Phytophthora and (23 taxa) Pythium (6 taxa) in the accessible part of the collection. The other 400 strains are deposited in the non-public section of the collection. The isolates were acquired from many various plant hosts including forest, ornamental, and fruit trees as well as other hosts from various other environments (riparian and forest stands, parks, urban greenery, orchards, public gardens, gardening centres, ornamental and forest nurseries etc.). The collection is operated by Marcela Mrázková (custodian), Markéta Hejná (molecular determination) and Liliya Fedusiv (technician).

• Methods of conservation
Individual isolates are maintained on agar slants (OA agar) in vials in a refrigerator at a temperature of about 12 ° C in four tubes. Their condition is regularly monitored and the strains are regularly re-inoculated at intervals of 1-2 years.

Overview of deposited taxa
1. Phytophthora ×alni (Brasier & S.A. Kirk) Husson, Ioos & Marçais
2. Phytophthora uniformis (Brasier & S.A. Kirk) Husson, Ioos & Aguayo
3. Phytophthora bilorbang Aghighi & T.I.Burgess
4. Phytophthora cactorum (Lebert & Cohn) J. Schröt.
5. Phytophthora cambivora (Petri) Buisman
6. Phytophthora cinnamomi Rands
7. Phytophthora citrophthora (R.E. & E.H. Smith) Leonian
8. Phytophthora cryptogea Pethybridge & Lafferty
9. Phytophthora gallica T. Jung & J. Nechwatal
10. Phytophthora gonapodyides (H.E. Petersen) Buisman
11. Phytophthora gregata T. Jung, Stukely & T.I. Burgess
12. Phytophthora hedraiandra De Cock & Man in ‚t Veld
13. Phytophthora lacustris Brasier, Cacciola, Nechwatal, Jung & Bakonyi
14. Phytophthora megasperma Drechsler
15. Phytophthora multivora P.M. Scott & T. Jung
16. Phytophthora palmivora (E.J. Butler) E.J. Butler
17. Phytophthora plurivora T. Jung & T.I. Burgess
18. Phytophthora polonica Belbahri, E. Moralejo, Calmin & Oszako
19. Phytophthora ramorum Werres, De Cock & Man in ‚t Veld
20. Phytophthora rosacearum (H.E. Petersen) Buisman
21. Phytophthora syringae (Kleb.) Kleb.
22. Phytophthora taxon Raspberry
23. Phytophthora taxon Walnut

1. Pythium citrinum B. Paul
2. Pythium helicoides Drechsler
3. Pythium chamaehyphon Sideris
4. Pythium intermedium de Bary
5. Pythium ultimum Throw.
6. Pythium vexans de Bary
The collection aims for the preservation of genetic diversity of important phytopathogenic and other oomycetes in the Czech Republic for scientific and other purposes (tests of pathogenicity and host resistance, testing and development of control measurements, reference studies in science and phytosanitary services, systematics, education, etc.)

Silva Tarouca research institute for landscape and ornamental gardening
Květnové nám. 391
252 43 Průhonice, Czech Republic
Ing. Marcela Mrázková, e-mail:, phone: +420 296 528 234

Culture Collection of Dairy and Bakery Contaminants
Sbírka mlékárenských a pekárenských kontaminantů (CCDBC)
MILCOM a.s., Soběslavská 841, 390 02 Tábor, Česká republika
Tel.: +420 733 591 080

Ing. Miloslava Kavková, Ph.D., vedoucí sbírky CCDBC
+420 723 679 012
Ing. Zuzana Dlouhá, kurátor sbírky CCDBC
+420 733 591 080

Czech Collection of Microorganisms
Information on collection
• History
The Czech Collection of Microorganisms (CCM) is a nonprofit organization established at the Faculty of Science, Masaryk University in 1963 and in the present serves as a specialized research and service division within the Department of Experimental Biology. The CCM was included into the National Programme (NPGZM) in 2019 with selected collection of approx. 200 bacterial strains in the frame of the project: „Microorganisms related to dairy technologies and products“.

• Characterisation of the CCM
The CCM serves as a depository for cultures of bacteria and filamentous fungi. The main goal of the CCM is to collect, maintain and preserve authentic cultures of living bacteria and filamentous fungi relevant to applied microbiology, biotechnology, education and those of general scientific interest A special set of strains is used as reference cultures for clinical, veterinary, food and water testing laboratories. The CCM public collection contains about 3400 bacterial strains covering more than 1700 species and about 800 fungal strains with more than 550 species. All cultures are available via a public catalogue of strains ( The CCM also maintains a unique collection of aquatic hyphomycetes containing approx. 500 strains (60 genera and 130 species) and a collection of staphylococcal bacteriophages and their propagation strains which was established in 2011. Since 1992, the CCM is an International Depositary Authority (IDA) for deposits of bacteria, yeasts and filamentous fungi for patent purposes under the Budapest Treaty. There are more than 200 patent strains of bacteria and fungi maintained in the CCM at present.

• Research activities
The major activities of the CCM focus on the preservation and distribution of microorganisms. The CCM continuously extents and diversifies its holdings (approx. 100 cultures per year) and contributes to the biodiversity conservation ex situ. Research activities funded in the frame of national research programmes are focused on the taxonomy of Gram-positive cocci and rods (mainly the phylum Firmicutes), Gram-negative rods (mainly the phyla Proteobacteria and Bacteroidetes) and aquatic hyphomycetes. Since 2008, the CCM has been actively involved in the microbiological exploration of Antarctic environment.

• Services
Cultures supply – CCM cultures are used for basic and applied research, industrial applications, education and general scientific interest. The cultures are distributed under the conditions of the CCM Material Transfer Agreement (MTA) following the OECD Best Practice Guidelines for BRCs and WFCC Culture Collections Guidelines. Holdings – the CCM holds strains of bacteria, fungi and bacteriophages in pure cultures, covering a wide range of applications (type or ex- type cultures derived from type, quality control strains for testing of media, assay of antibiotics, vitamins and amino acids, testing of disinfectants, commercial diagnostic kits, enzymes and secondary metabolites producing strains, biodegrading strains for biotechnology, etc.). Safe keeping – the CCM offers a long-term preservation of viable and genetically unaltered customers‘ bacterial and fungal cultures under optimal conditions. Deposited cultures remain the property of the depositor. The CCM does not provide these cultures nor any information about them to third parties without the owner’s written permission. Freeze-drying – the CCM is able to prepare freeze-dried ampoules to customers who require keeping their cultures for a long time in their own laboratory. Identification of microorganisms – the CCM offers isolation and purification of cultures from provided samples and identification of bacteria and fungi by using phenotypic and molecular techniques. Consultancy – the CCM provides advisory and consultation services on enrichment, isolation, identification and preservation of microorganisms.

• Maintenance of CCM cultures
CCM cultures are preserved mainly as freeze-dried conserves (lyophilisation), also frozen in liquid nitrogen at -196 °C and deep-frozen at -70 °C.

• International cooperation
The CCM cooperates with other culture collections within the Czech Republic as well as abroad. It is recognized by the World Federation for Culture Collections (member No. 65) and is also a member of the European Culture Collections’ Organisation and the Federation of Czech and Slovak Culture Collections.

• Quality system and in-house rules
The CCM introduced the System of Quality Management and was certified according to the ČSN EN ISO 9001:2001 standards on June 22, 2006. Recertification according to the ČSN EN ISO 9001:2015 has been validated since June 4, 2017. All laboratory procedures follow the standard operation procedures complying with the Good Laboratory Practice rules. All information concerning CCM strains and their preservation and distribution is unified in a database based on the MINE (Microbial Information Network Europe) format.

Application of CCM cultures, users
CCM strains are used primarily for the diagnostic purposes as well as for research and education in the Czech Republic or abroad. The most frequent users are public health institutions and laboratories of veterinary medicine but also other research institutions, universities, agricultural laboratories or food and water testing laboratories.

Czech Collection of Microorganisms
Department of Experimental Biology
Faculty of Science,
Masaryk University Kamenice 5, buiding A25,
625 00 Brno,
Czech Republic
Tel.: +420-549496922

Web pages

Responsible person:
Assoc. Prof. RNDr. Ivo Sedláček, CSc.,
Head of the CCM, +420-549496922

Other workers Ing. Sylva Koudelková, Ph.D.,
Curator of bacterial collection

Ing. Monika Laichmanová, Ph.D.,
Curator of fungal collection