The Czech National Programme on Conservation and Utilization of Microbial Genetic Resources Important for Agriculture

Introduction Animal Pathogenic Microorganisms Phytopathogenic Microorganisms Olomouc Hop Pathogens Industrially Utilizable Microorganisms Dairy Microorganisms
Brewery Microorganisms Phytopathogenic Viruses Fruit Trees and Small Fruit Viruses Potato Viruses Ornamental Plant Viruses Phytopathogenic Bacteria
Rhizobia Rusts and Powdery Mildew Fungi CCF Phytopathogenic Fungi CRI Phytopathogenic Oomycetes Edible and Medicinal macromycetes
Basidiomycetes Invertebrate Crop Pests and their Natural Enemies Stored-Product Pests, Mites and Fungi Food Technologies CCM Dairy and Bakery Contaminants Clavicipitaceae


Collection of Biotrophic Fungi


•    History
Determination of physiologic races of wheat rusts started in the sixties of the last century. Results were periodically published in scientific journals (Genetika a šlechtění, at present Czech Journal of Genetics and Plant Breeding or in Ochrana rostlin, at present Plant Protection Science). Results of race surveys were discussed with wheat breeders and applied in the wheat breeding for rust resistance. Since that time determination of rust races (pathotypes) is being carried out till now and isolates are stored.

•    Characterization of collection
  The collection comprises 826 leaf rust isolates, 29 stem rust isolates, 2 proveniences of yellow rust and 15 isolates of powdery mildew.
•    Activities, services
The rust isolates are used in the tests for resistance in the field as well as in the greenhouse. Rust inoculum is used by domestic and also foreign breeders and institutions.

•    Methods of culture maintenance
Determined rust isolates are kept in collections as urediospores. Powdery mildew is kept on leaf segments. Isolates most frequently applied are kept in a refrigerator at 5°C, the core collection is kept at ultra low temperature at -85°C.
•    Systems of quality
Determination of rust isolates is carried our on international sets of differentials. It is based on determination of single pustule isolates. Approved methods are used for storing the rust and powdery mildew isolates.

•    Involvement to national and international organizations/platforms
Rust isolates have been used in international scientific collaboration (Australia, U.K., France, Hungary, and Slovakia). At the domestic level accessions of the collection are used by plant breeders and by the Central Institute for Supervising and Testing in Agriculture and a similar institution in Slovakia.


•    utilization of strains
Isolates are used in the wheat breeding as well as resistance testing both in the Czech and Slovak Republics. They are used by the Central Institute for Supervising and Testing in Agriculture and the. They are also used in the research work on national and international level.

•    users, clients
Plant breeding companies
Central Institute for Supervising and Testing in Agriculture
Central Controlling and Testing Institute in Agriculture in Slovakia
Foreign scientific institutes


Crop Research Institute
Division of Plant Genetics, Breeding and Product Quality
Drnovská 507
161 06 Praha 6 – Ruzyně
Czech Republic

Mgr. Alena Hanzalová, Ph.D. (curator)
e-mail:, tel: 233022243
Ing. P. Bartoš, DrSc

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