The Czech National Programme on Conservation and Utilization of Microbial Genetic Resources Important for Agriculture
|Introduction||Animal Pathogenic Microorganisms||Phytopathogenic Microorganisms Olomouc||Hop Pathogens||Industrially Utilizable Microorganisms||Dairy Microorganisms|
|Brewery Microorganisms||Phytopathogenic Viruses||Fruit Trees and Small Fruit Viruses||Potato Viruses||Ornamental Plant Viruses||Phytopathogenic Bacteria|
|Rhizobia||Rusts and Powdery Mildew||Fungi CCF||Phytopathogenic Fungi CRI||Phytopathogenic Oomycetes||Edible
and Medicinal macromycetes
|Basidiomycetes||Invertebrate Crop Pests and their Natural Enemies||Stored-Product Pests, Mites and Fungi||Food Technologies CCM||Dairy and Bakery Contaminants||Clavicipitaceae|
The collections of potato pathogenic viruses and viroids were in Potato Research Institute in Havličkuv Brod founded and maintained since seventies of the last century. Methods of long-term conservation and stabilization of viruses (procedures of freez-drying and drying over strong desicants) were solved in research projects dealing with viruses occurring on potato in CR. Afterwards experimental utilization of tissue culture technique for maintenance of isolates in in vitro conditions was devised. The originál collection included altogether 231 isolates of six main potato viruses.
• Characterization of collection
The majority of isolates are currently maintained in aseptic in vitro conditions and regularly restored using nodal segment of plantlets. Original potato cultivars infected with particular virus or viroid isolate are for long term storage maintained in vitro in microtubers. The plant segments are kept on semi-solid agar media and special environmental conditions and chemical components are utilized. Before transmission of isolates into in vitro conditions as well as at regular intervals during their maintenance the isolates are checked using serological, biological and selected molecular diagnostic methods, including sequincing of their nucleic acid.
At present 544 isolates of main potato viruses, devided into seven sub-sets are mainatained.
Ptato leafroll virus (PLRV) - 61 isolates in vitro on originál potato cultivars
Potato virus Y (PVY) – 12 isolates on tobacco plants and 100 isolates on potato cultivars
Potato virus A (PVA) – 5 isolates on tobacco plants and 25 isolates on potato cultivars
Potato virus M (PVM) – 43 isolates on potato cultivars
Potato virus X (PVX) – 27 isolates on potato cultivars
Potato virus S (PVS) – 262 isolates on potato cultivars
Potato virus V (PVV) – 1 isolate on potato cultivar
Potato Mop-Top virus (PMTV) – 5 isolates on potato cultivars
Potato Aucuba mosaic virus (PAMV) – 2 isolates on potato cultivars
Potato Rough Dwarf virus (PRDV) – 1 isolate on potato cultivar
Tobacco rattle virus (TRV) – 1 isolate on potato cultivar
Potato Spindle Tuber Viroid (PSTVd) – 2 potato isolates on potato cultivars and 7 isolates from ornamental plants.
• Activities, services
Individual isolates of the collection are used in a number of solved research projects, and are on request supply to all the laboratories of large scale testing as the positive controls for ELISA and further laboratory diagnostic tests.
• Methods of culture maintenance
In in vitro conditions the isolates are kept in aseptic conditions on semi-solid artificial medium and depending on initial host plants they are restored using nodal segment of plantlets. For long term storage the technique of micro tubers has been incorporated. Testing of individual isolates has been performed and repeated in regular intervals and positive isolates are re-introduced into collection.
Contact:Potato Research Institute Havlíčkův Brod L.t.d.
58001 Havlíčkův Brod
Ing. Martin Kmoch, Ph.D. (curator)
tel.: 569 466 231
webové stránky: www.vubhb.cz