The Czech National Programme on Conservation and Utilization of Microbial Genetic Resources Important for Agriculture
|Introduction||Animal Pathogenic Microorganisms||Phytopathogenic Microorganisms Olomouc||Hop Pathogens||Industrially Utilizable Microorganisms||Dairy Microorganisms|
|Brewery Microorganisms||Phytopathogenic Viruses||Fruit Trees and Small Fruit Viruses||Potato Viruses||Ornamental Plant Viruses||Phytopathogenic Bacteria|
|Rhizobia||Rusts and Powdery Mildew||Fungi CCF||Phytopathogenic Fungi CRI||Phytopathogenic Oomycetes||Edible
and Medicinal macromycetes
|Basidiomycetes||Invertebrate Crop Pests and their Natural Enemies||Stored-Product Pests, Mites and Fungi||Food Technologies CCM||Dairy and Bakery Contaminants||Clavicipitaceae|
Information on collection• History
The Czech Collection of Microorganisms (CCM) is a nonprofit organization established at the Faculty of Science, Masaryk University in 1963 and in the present serves as a specialized research and service division within the Department of Experimental Biology. The CCM was included into the National Programme (NPGZM) in 2019 with selected collection of approx. 200 bacterial strains in the frame of the project: „Microorganisms related to dairy technologies and products“.
• Characterisation of the CCM
The CCM serves as a depository for cultures of bacteria and filamentous fungi. The main goal of the CCM is to collect, maintain and preserve authentic cultures of living bacteria and filamentous fungi relevant to applied microbiology, biotechnology, education and those of general scientific interest A special set of strains is used as reference cultures for clinical, veterinary, food and water testing laboratories. The CCM public collection contains about 3400 bacterial strains covering more than 1700 species and about 800 fungal strains with more than 550 species. All cultures are available via a public catalogue of strains (www.sci.muni.cz/ccm). The CCM also maintains a unique collection of aquatic hyphomycetes containing approx. 500 strains (60 genera and 130 species) and a collection of staphylococcal bacteriophages and their propagation strains which was established in 2011. Since 1992, the CCM is an International Depositary Authority (IDA) for deposits of bacteria, yeasts and filamentous fungi for patent purposes under the Budapest Treaty. There are more than 200 patent strains of bacteria and fungi maintained in the CCM at present.
• Research activities
The major activities of the CCM focus on the preservation and distribution of microorganisms. The CCM continuously extents and diversifies its holdings (approx. 100 cultures per year) and contributes to the biodiversity conservation ex situ. Research activities funded in the frame of national research programmes are focused on the taxonomy of Gram-positive cocci and rods (mainly the phylum Firmicutes), Gram-negative rods (mainly the phyla Proteobacteria and Bacteroidetes) and aquatic hyphomycetes. Since 2008, the CCM has been actively involved in the microbiological exploration of Antarctic environment.
Cultures supply - CCM cultures are used for basic and applied research, industrial applications, education and general scientific interest. The cultures are distributed under the conditions of the CCM Material Transfer Agreement (MTA) following the OECD Best Practice Guidelines for BRCs and WFCC Culture Collections Guidelines. Holdings - the CCM holds strains of bacteria, fungi and bacteriophages in pure cultures, covering a wide range of applications (type or ex- type cultures derived from type, quality control strains for testing of media, assay of antibiotics, vitamins and amino acids, testing of disinfectants, commercial diagnostic kits, enzymes and secondary metabolites producing strains, biodegrading strains for biotechnology, etc.). Safe keeping - the CCM offers a long-term preservation of viable and genetically unaltered customers' bacterial and fungal cultures under optimal conditions. Deposited cultures remain the property of the depositor. The CCM does not provide these cultures nor any information about them to third parties without the owner's written permission. Freeze-drying - the CCM is able to prepare freeze-dried ampoules to customers who require keeping their cultures for a long time in their own laboratory. Identification of microorganisms - the CCM offers isolation and purification of cultures from provided samples and identification of bacteria and fungi by using phenotypic and molecular techniques. Consultancy – the CCM provides advisory and consultation services on enrichment, isolation, identification and preservation of microorganisms.
• Maintenance of CCM cultures
CCM cultures are preserved mainly as freeze-dried conserves (lyophilisation), also frozen in liquid nitrogen at -196 °C and deep-frozen at -70 °C.
• International cooperation
The CCM cooperates with other culture collections within the Czech Republic as well as abroad. It is recognized by the World Federation for Culture Collections (member No. 65) and is also a member of the European Culture Collections’ Organisation and the Federation of Czech and Slovak Culture Collections.
• Quality system and in-house rules
The CCM introduced the System of Quality Management and was certified according to the ČSN EN ISO 9001:2001 standards on June 22, 2006. Recertification according to the ČSN EN ISO 9001:2015 has been validated since June 4, 2017. All laboratory procedures follow the standard operation procedures complying with the Good Laboratory Practice rules. All information concerning CCM strains and their preservation and distribution is unified in a database based on the MINE (Microbial Information Network Europe) format.
of CCM cultures, users
CCM strains are used primarily for the diagnostic purposes
as well as for research and education in the Czech Republic
or abroad. The most frequent users are public health
institutions and laboratories of veterinary medicine but
also other research institutions, universities, agricultural
laboratories or food and water testing laboratories.
Analysis of fatty acids
Diversity pigment strain bacteria
Preservation strain in liquid nitrogen
Macroscopic morphology threadlike fungi
Storage culture in deep-frozen box