The Czech National Programme on Conservation and Utilization of Microbial Genetic Resources Important for Agriculture


•    History
The collection of brewer's yeast was established in 1946 as part of a collection of yeasts and yeast-like microorganisms under the leadership of Dr. Kocková-Kratochvílová, and has operated independently since 1953. Since 1964 the collection has been a member of the Federation of Czech and Slovak Collections of Microorganisms and is internationally registered as RIBM under the number 655. By focusing on the production strains of brewing yeast it is unique in Central and Eastern Europe. In 1996 the brewer's yeast collection became (as one of very specifically targeted and industrially applicable collections) part of the "National Program of the Gene Pool of Microorganisms and Small Animals of Economic Importance and their Use in Reference Diagnostics." This project is also supported by the Czech government in the form of a grant by the Ministry of Agriculture. The program is led by the Council of Microbial Genetic Resources, which monitors the mandatory retention of a functional existence of collections of microorganisms of a corresponding character.

•    Characterization of collection
RIBM collection includes two separate collections, collections of brewer's yeast and collection of bacteria and wild and wine yeast. The collection currently includes a total of 308 strains of yeasts and bacteria. The most important part of the collection consists of a collection of 118 strains of brewer´s yeast Saccharomyces cerevisiae and S. pastorianus, collected continuously since 1953 from Czech and foreign breweries. A collection of wild and wine yeasts contains 79 strains classified into genera Saccharomyces, Torulaspora, Zygosaccharomyces, Dekkera, Williopsis, Pichia, Schizosaccharomyces, Saccharomycodes, Candida, Kloeckera and Rhodotorula. Collection of bacteria houses 108 strains of Lactobacillus, Leuconostoc, Pediococcus, Tetragenococcus, Lactococcus and three strains of genus Pectinatus.

•    Methods of culture maintenance
Collections of brewing and wild yeasts are kept on agar slants under paraffin-covered cotton wool plugs and simultaneously on wort agars overlayered with sterile paraffin oil separately in a refrigerator. These best ways of keeping the cultures allow the rapid delivery to a customer of a strain in an active state on an agar slant or fermenting in 1.5 liters of wort, which facilitates the transfer of the production strain into production. Since 2006 strains of brewer's yeast are stored in cryo-tubes with protective medium in liquid nitrogen at -196 ° C. Storing in liquid nitrogen (cryopreservation) is considered optimal for the long term preservation of yeast in a viable state. Lactic acid bacteria are stored in parallel freeze-dried in skimmed milk in liquid nitrogen.


Use of the collection:
Collection strains are primarily used for research projects of RIBM and other research organizations (MSM6019369701, 1M0570, 2B08022 2A-2TP1 / 031, FI-IM5 / 067, QF3299, QI91B226 etc.). The strains are also used for teaching and diploma and doctoral works at universities.


Research Institute of Brewing and Malting
Lípová 15
120 44 Prague 2 

•    curator
RNDr. Dagmar Matoulková, Ph.D.
  phone.: 224 900 132
•    others
Ing. Petra Kubizniaková 
phone.: 224 900 152


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