The Czech National Programme on Conservation and Utilization of Microbial Genetic Resources Important for Agriculture

Introduction Animal Pathogenic Microorganisms Phytopathogenic Microorganisms Olomouc Hop Pathogens Industrially Utilizable Microorganisms Dairy Microorganisms
Brewery Microorganisms Phytopathogenic Viruses Fruit Trees and Small Fruit Viruses Potato Viruses Ornamental Plant Viruses Phytopathogenic Bacteria
Rhizobia Rusts and Powdery Mildew Fungi CCF Phytopathogenic Fungi CRI Phytopathogenic Oomycetes Edible and Medicinal macromycetes
Basidiomycetes Invertebrate Crop Pests and their Natural Enemies Stored-Product Pests, Mites and Fungi Food Technologies CCM Dairy and Bakery Contaminants Clavicipitaceae


Culture Collection of Basidiomycetes Important for Agriculture (CCBAS-A)
                             (an integral part of the Culture Collection of Basidiomycetes (CCBAS))


•    History
Culture Collection of Basidiomycetes Important for Agriculture (CCBAS-A) is an integral part of the mother Culture Collection of Basidiomycetes (CCBAS), which was established in 1959 at the Institute of Microbiology AS CR in Prague. Since 2003 the CCBAS-A is incorporated in the National Programme of Protection and Utilization of Genetic Resources of Microorganisms and Tiny Organisms Economically Significant for Agriculture. At present it includes 346 strains of basidiomycetes belonging to 168 species, mostly wood-rotting, and is continually extended by adding new verified strains.

•    Characterization of Collection
potential to decompose polutants (organic compound) with their enzymes
metabolize pesticides thanks to extracellular enzyme lacasa
thus help with bioremediation of contaminated soil
332 isolates

•    Activities/research activities
The main goal of the work of the collection staff is to develop alternative and to optimize current procedures of cryopreservation and other methods of maintenance of fungal cultures. These methods are then verified and introduced into practice. A new original method of cryopreservation of fungal cultures on perlite was developed and verified in our laboratory.  This method is now used for the preservation of fungi not only in our, but also in many other collections in the world. Our cultures can serve as a reference material for further taxonomic, phylogenetic and other studies including studies of their biotechnologically exploitable properties. They are plentifully utilized by home and foreign research scientists. Another activities lie in consultations regarding cultivation, physiology and genetics of basidiomycetes and publishing of the achieved results in impacted international journals. Dr. Homolka takes part in home and international conferences and congresses and gives lectures at the Faculty of natural sciences (Essentials of physiology and genetics of filamentous fungi). He is also engaged in the training the staff of the collections in database operation.

•    Methods of culture maintenance
The cultures are maintained by two independent principal methods (subculturing on solid agar media and cryopreservation on perlite) to preserve their quality and quantity. The crucial characteristics of the cultures are recorded in the database of the National Programme. The cultures on perlite can be distributed directly without reinoculation and reused for successive inoculations. They also turned out to be a good substitute for agar cultures in long-term maintenance of fungi.
The viability of the cultures and their crucial characteristics (macro- and micromorphology, growth rate, purity) is tested every year in case of agar cultures and every second to fifth year in case of perlite cultures. If needed, the specific characteristics are tested, e.g. enzymatic or genetic ones.
The data on cultures and collection operation are stored in a local operation database (developed especially for this purpose) and also in a central database of the National Programme.

•    Database
involvement to national and international organizations/platforms
CCBAS is a member of Federation of Czechoslovak Collections of Microorganisms (FCCM) and World Federation of Culture Collections (WFCC), where it is listed under the number 558. In the frame of the international cooperation and information exchange, our collection provides the interested persons with the data on the maintained basidiomycete cultures (see the databases of NP, FCCM or WFCC) and on the basis of order also cultures to abroad. CCBAS cooperates with home and renowned foreign culture collections (e.g. CBS at Utrecht or MUCL at Louvain-la-Neuve) and universities (e.g. at Wageningen or at Oslo).
As mentioned above, the strains are used predominantly for noncommercial research and teaching purposes.



Institute of Microbiology of the Academy of Sciences of the Czech Republic
Laboratory of Environmental of Microbiology
Vídeňská 1083
142 20 Prague 4
Czech Republic

•    head and curator
RNDr. Ivana Eichlerová, PhD,
•    others
Ing. Petra Dobiášová

tel.: 241 062 397
fax: 241 062 384

web CCBAS:

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