| Autoři: | Kameník, Z., Kopecký, J., Marečková, M., Ulanová, D., Novotná, J., Pospíšil, S., Olšovská, J. |
| Název: | HPLC-fluorescence detection method for determination of key intermediates of the lincomycin biosynthesis in fermentation broth. |
| Abstrakt: | The biosynthetic pathway of the clinically important antibiotic lincomycin is not known in details. The precise knowledge of the lincomycin biosynthesis is a prerequisite for generation of improved derivatives by means of combinatorial genetics. Methods allowing determination of the key intermediates are very important tools of the pathway investigation. Two new high-performance liquid chromatography methods with fluorescence detection for determination of lincomycin precursors in fermentation broth of Streptomyces lincolnensis and its lincomycin nonproducing mutants were developed. The first one enables simultaneous analysis of methylthiolin-cosamide (MTL) and . |
| Klíčová slova: | Prekurzory linkomycinu; o-Ftaldialdehyd; 4-Chloro-7-nitrobenzofurazan; HPLC; Fluorescenční detekce |
| Rok: | 2009 |
| Časopis: | Analytical and Bioanalytical Chemistry |
| Ročník časopisu: | 393 |
| číslo časopisu: | 6-7 |
| Stránky: | 1779 - 1787 |
| Jazyk: | Angličtina |
| Kategorie: | Články v impaktovaných časopisech |
| Wok: | |
| DOI: | 10.1007/s00216-009-2605-3 |
| PDF: | |
