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PROJECT AGRI GEN RES 050 (EURALLIVEG)

“Vegetatively propagated alliums, Europe’s core collection with higher maintenance safety at lower costs and better health conditions”

FINAL Meeting

1 - 2 March 2011

Crop Research Institute (CRI) at Prague-Ruzyne, Czech Republic.

(Arrival day 28 February, Departure day 3 March)

Agenda

 Day 1 (1. March 2011)

 

 General Part

8:30 – 8.40                 Welcome address by director of Crop Research Institute  (František Kocourek)

8:40 – 8:50                 Opening by the Project Coordinator (E. R. Joachim Keller)

8:50 – 9:20                 The EU policy context and perspectives for plant genetic resources conservation and use (Olivier Diana)

9:20 – 9:50                 Genetic Establishment and operation of the European Collection with an emphasis on vegetatively propagated Allium (Jan Engels)

9:50 - 10:20                The Allium Working Group in the present phase and the stage of development of the European Allium Database (Dave Astley)

 10:20 – 10:50             Coffee break

10:50 – 11:20             Presentation of the main subjects, milestones and deliveries of the project (E. R. Joachim Keller)

 Special Part - The Work Packages of the EURALLIVEG Project

11:20 – 12:00             Dokumentation (E. R. Joachim Keller / Christine Zanke)

- The Core Collection – Criteria for its selection

- Presentation of the written documentation and the Gatersleben Allium Database as an example for further activities

- Presentation of the garlic and shallot accessions using some examples 

12:00 – 13:00             Molecular Duplicate Search - Approaches and Problems (Christine Zanke/ Chris Kik)

- Probe sampling, DNA isolation and data processing at Gatersleben

- Evaluation, conclusions and further strategy

 13:00 – 14:30             Lunch break

14:30 15:15             Press Conference with Czech Journalists  

15:15 16:45            Cryopreservation (Christine Zanke, Jiří Zámečník, Teresa Kotlińska)

- Special results of the Gatersleben laboratory (P0; Christine Zanke)

- Special results of the Prague laboratory (P1; Renata Kotková)

- Special results of the Skierniewice laboratory (P2; Marta Olas-Sochacka)

- Synthesis of the cryopreservation results and Establishment of the Tripartite Cryobank of the European Garlic Collection including the exchange of Safety duplicates (Jiří Zámečník)

Comment: 20 min time for each person

16:45 – 17:05             Coffee break

17:05 – 17:45             Virus elimination (Vito Miccolis)

- Special results of the Potenza Laboratory (P3; Luciana Altieri)

- Learning from sample sending strategies

- Field test on performance of virus-free material

17:45 – 18:25             Coordination (Christine Zanke / E. R. Joachim Keller)

- The project website

- Evaluation of annual and small technical meetings

- Conclusions

 18:45 “Not only garlic tasting“  - Congress hall lobby

 ______________________________________________________________________

 

 

 

 

Day 2 (2. March 2011)

 

 Workshop on Aspects of Germplasm Preservation of Allium

 

8:00 – 8:20                DNA Markers in Allium evaluation   (Jaroslava Ovesná)

8:20 – 8:40                The possibilities of the detection of Allium viruses using real-time PCR  (Leona Svobodová)

8:40 – 9:00                Cryopreservation as a component in a complex genebank - cost implications (E.R. Joachim Keller / Christine Zanke)

9:00 – 9:20                Cryopreservation of young inflorescence bases in bolting garlic for germplasm storage – a small AEGIS project (Christine Zanke)

9:20 – 9:30                Technical break to prepare the preparation and demonstration tools

9:30 – 10:30                Practical Demonstrations

                                            -  Preparation of explants for cryopreservation and virus elimination

10:30 – 11:00             Coffee break  

11:00 – 12:30               Discussions

- Role of endophytes and other factors

- Use of other sources for cryopreservation

- Virus indexing by ELISA compared with new tendencies such as PCR

- Discussing new chances for virus elimination by the cryo knife method

12:30 – 12:50             Cryopreservation – its potential and application strategies in the Czech National Programme on Conservation and Utilization of Plant Genetic Resources (Ladislav Dotlačil)

12:50 – 13:00             Impact of the project on garlic and shallot breeding using the Czech example (Helena Stavělíková)

13:00 – 14:30             Lunch break

14:30 – 15:15             Future Collaboration

- Presentation of activities on garlic and shallot and statements of invited guests from Portugal and Spain concerning future collaboration to extend the European Garlic and Shallot collection to a broader covering (Ana Barata and Francisco Mansilla)

- Tendencies to expand and coordinate the European Allium collection beyond garlic and shallot (E. R. Joachim Keller)

15:15 – 16:00             Final conclusion 

17:00 Departure from the Meritum hotel to the sight-seeing baroque monastery st. Margaret and Romanesque undercroft from 11th century 

18:00 Farewell Dinner

 ~       Departure back to the hotel Meritum